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CyFlow™ CD3 PE-Cy7

CyFlow™ CD3 PE-Cy7
Aplicación: Citometría de Flujo
Isotype: IgG1
Target Species: Human, Non-Human Primates
Alternative Name: Leu4, T3
Antigen: CD3
Pico de Emisión: 785 nm
Format/Fluorochrome: PE-Cy7
Excitation Maximum: 496 nm, 565 nm
Clonality: monoclonal
Láser: Azul, Green, Yellow
Campo de aplicación: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Clone: SK7
Número de pedido BQ006366
HLDA Workshop HLDA II—WS Code T118 HLDA III—WS Code T492 Quantity 100 tests Volume... more
Información del producto "CyFlow™ CD3 PE-Cy7"
HLDA WorkshopHLDA II—WS Code T118 HLDA III—WS Code T492
Quantity100 tests
Volume0.4 mL
ImmunogenHuman thymocytes
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Technical Data Sheet

Instruction for Use

ECDoC

Certificate Of Analysis

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