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CyFlow™ Bcl2 PE

CyFlow™ Bcl2 PE
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green, Yellow
Source Species: Mouse
Format/Fluorochrome: PE
Excitation Maximum: 496 nm, 565 nm
Emission Maximum: 576 nm
Isotype: IgG1
Field of Interest: Apoptosis
Antigen: Bcl2
Clone: Bcl-2/100
Order number: AE483629
Quantity 100 tests Volume 2.0 mL Immunogen Synthetic peptide corresponding to the... more
Product information "CyFlow™ Bcl2 PE"
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green, Yellow
Source Species: Mouse
Format/Fluorochrome: PE
Excitation Maximum: 496 nm, 565 nm
Emission Maximum: 576 nm
Isotype: IgG1
Field of Interest: Apoptosis
Antigen: Bcl2
Clone: Bcl-2/100
Quantity100 tests
Volume2.0 mL
ImmunogenSynthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Background InformationBcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.